LOCUS       pLV-[hsa-miR-374b]        9124 bp    DNA     circular     20-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7045..7905
                     /label=Amp(R)
     promoter        8106..8646
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5650..5884
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6459..6914)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5956..6086
                     /label=SV40 pA
     misc_feature    6087..6300
                     /label=SV40 ori
     misc_feature    4971..5567
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3589
                     /label=intron
     CDS             3636..4961
                     /label=rPuro (RFP Puro)
     misc_feature    3104..3109
                     /label=BamH I / Bgl II
     misc_RNA        3110..3379
                     /label=hsa-mir-374b precursor
BASE COUNT     2167 a      2373 c      2468 g      2116 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatctt gggacaccca 
     3121 gcacgtgaca aaaacaacag cagaccctgg tgcctgggag gacttcatgg atccagcagc 
     3181 aacctggagt ggtgctcctc tgaagaaatc ctactcggat ggatataata caacctgcta 
     3241 agtgtcctag cacttagcag gttgtattat cattgtccgt gtctatggct ctcgtctacc 
     3301 agactttaaa ttccctaagg gcaaggacag tgccttactc atctttgtat tcacagtgcc 
     3361 taatccggtg cacattgtag ctagctcgag cttttggagt acgtcgtctt taggttgggg 
     3421 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc 
     3481 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt 
     3541 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa 
     3601 actacccctc tagagtcgag ctaccggtcg ccaccatggt gagcaagggc gaggaggata 
     3661 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg 
     3721 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg 
     3781 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc 
     3841 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga 
     3901 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg 
     3961 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc 
     4021 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg 
     4081 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga 
     4141 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca 
     4201 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc 
     4261 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg 
     4321 gcggcatgga cgagctgtac aaggacccac cggtcgccac catgaccgag tacaagccca 
     4381 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt 
     4441 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca 
     4501 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg 
     4561 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg 
     4621 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc 
     4681 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca 
     4741 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg 
     4801 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca 
     4861 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg 
     4921 accgcgcgac ctggtgcatg acccgcaagc ccggtgcctg agcggccgcg tcgacaatca 
     4981 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 
     5041 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 
     5101 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 
     5161 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 
     5221 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 
     5281 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 
     5341 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 
     5401 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 
     5461 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 
     5521 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg 
     5581 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 
     5641 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt 
     5701 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
     5761 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
     5821 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     5881 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 
     5941 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     6001 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     6061 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc 
     6121 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
     6181 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
     6241 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc 
     6301 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 
     6361 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 
     6421 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt 
     6481 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     6541 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     6601 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     6661 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     6721 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     6781 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 
     6841 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt 
     6901 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 
     6961 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 
     7021 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 
     7081 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 
     7141 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 
     7201 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 
     7261 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 
     7321 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 
     7381 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 
     7441 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 
     7501 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 
     7561 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 
     7621 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 
     7681 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 
     7741 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 
     7801 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 
     7861 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 
     7921 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 
     7981 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 
     8041 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     8101 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     8161 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     8221 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     8281 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     8341 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     8401 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     8461 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     8521 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     8581 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     8641 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     8701 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     8761 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     8821 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 
     8881 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 
     8941 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 
     9001 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc 
     9061 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag 
     9121 ctgc 
//
